RESUMEN
Las pérdidas reproductivas constituyen una causa importante de pérdida económica en el ganado bovino, aunque en más del 50% de los casos la etiología es desconocida. Las especies de la familia Chlamydiaceae han sido asociadas con abortos en bovinos y otras espeChlamydia abortus; cies animales, pero no existen datos al respecto en la República Argentina. El objetivo de este trabajo fue determinar la presencia de Chlamydia spp. y de Chlamydia abortus en pérdidas reproductivas de ganado bovino en La Pampa, Argentina. Se estudiaron 251 muestras provenientes de abortos y mortinatos. Se realizó PCR en tiempo real para la detección de la familia Chlamydiaceae y ArrayTube para la identificación de las especies presentes. Se detectó ADN de la familia Chlamydiaceae en 12 muestras (4,78%); el 83,33% (10/12) correspondió a abortos y el 16,66% (2/12) a mortinatos. El análisis por ArrayTube detectó C. abortus en 5 muestras (1,99% del total, 41,67% de las muestras con detección de Chlamydiaceae). Este trabajo presenta la primera confirmación de la presencia de ADN de diversas especies de Chlamydiaceae (incluida C. abortus) en muestras de pérdidas reproductivas de ganado bovino en Argentina. El valor de prevalencia hallado (4,78%) debe ser tomado como un valor basal, debido al tipo de muestras estudiadas. Se halló material genético de Chlamydiaceae que no coincidió con ninguna de las especies conocidas; esto podría deberse a variantes intraespecie o a especies autóctonas aún no descriptas. Es necesario avanzar en el estudio de la infección por estas bacterias en el ganado bovino de Argentina para conocer su dimensión y analizar su impacto económico y zoonótico, y también para planear medidas de prevención y control.
Reproductive losses linked to an infectious etiology in bovine cattle are a major economic concern worldwide. In Argentina, more than 50% of abortion cases have unknown causes. Species belonging to Chlamydiaceae family are frequent etiologic agents of abortion around the world; however, there is yet no information on their prevalence in Argentina. The objective of this work was to identify Chlamydia spp., and particularly C. abortus in reproductive losses from bovine cattle in La Pampa, Argentina. Real time PCR targeting Chlamydiaceae-specific DNA fragments was performed on 251 samples obtained from bovine abortions and stillborns, and ArrayTube was used for species identification on positive samples. Chlamydiaceae DNA was detected in 12 samples of aborted fetuses (4.78%), 83.33% (10/12) accounting for abortions and 16.66% (2/12) for stillborns. C. abortus was detected by ArrayTube in 5 cases (1.99% of all samples, and 41.67% of Chlamydiaceae positive samples). This study shows the first detection of Chlamydiaceae and C. abortus DNA on reproductive losses of bovine cattle in Argentina, and the described prevalence value (4.78%) should be taken as baseline value due to the type of samples analyzed. Detection of genetic material from Chlamydiaceae not matching any of the studied species could be due to intraspecies variants or local species not yet described. Further research on Chlamydia infections in bovine cattle in Argentina is imperative to describe their range, to analyze their economic and zoonotic implications and to make recommendations about prevention and control measures.
Asunto(s)
Animales , Bovinos , Femenino , Embarazo , Infecciones por Chlamydia , Enfermedades de los Bovinos , Chlamydia , Argentina , Reproducción , Infecciones por Chlamydia/diagnóstico , Infecciones por Chlamydia/veterinaria , Enfermedades de los Bovinos/diagnóstico , Chlamydia/aislamiento & purificaciónRESUMEN
Reproductive losses linked to an infectious etiology in bovine cattle are a major economic concern worldwide. In Argentina, more than 50% of abortion cases have unknown causes. Species belonging to Chlamydiaceae family are frequent etiologic agents of abortion around the world; however, there is yet no information on their prevalence in Argentina. The objective of this work was to identify Chlamydia spp., and particularly C. abortus in reproductive losses from bovine cattle in La Pampa, Argentina. Real time PCR targeting Chlamydiaceae-specific DNA fragments was performed on 251 samples obtained from bovine abortions and stillborns, and ArrayTube was used for species identification on positive samples. Chlamydiaceae DNA was detected in 12 samples of aborted fetuses (4.78%), 83.33% (10/12) accounting for abortions and 16.66% (2/12) for stillborns. C. abortus was detected by ArrayTube in 5 cases (1.99% of all samples, and 41.67% of Chlamydiaceae positive samples). This study shows the first detection of Chlamydiaceae and C. abortus DNA on reproductive losses of bovine cattle in Argentina, and the described prevalence value (4.78%) should be taken as baseline value due to the type of samples analyzed. Detection of genetic material from Chlamydiaceae not matching any of the studied species could be due to intraspecies variants or local species not yet described. Further research on Chlamydia infections in bovine cattle in Argentina is imperative to describe their range, to analyze their economic and zoonotic implications and to make recommendations about prevention and control measures.
Asunto(s)
Enfermedades de los Bovinos , Infecciones por Chlamydia , Chlamydia , Animales , Argentina , Bovinos , Enfermedades de los Bovinos/diagnóstico , Chlamydia/aislamiento & purificación , Infecciones por Chlamydia/diagnóstico , Infecciones por Chlamydia/veterinaria , Femenino , Embarazo , ReproducciónRESUMEN
Las amebas de vida libre del género Acanthamoeba incluyen cepas patógenas y no patógenas que actualmente se encuentran clasificadas en 18 genotipos diferentes (T1-T18). Se llevó a cabo un relevamiento para determinar la presencia de cepas de Acanthamoeba en agua de bebida para consumo ganadero en la provincia de La Pampa, Argentina. Las muestras fueron sembradas en agar no nutritivo. La identificación de cepas de Acanthamoeba se realizó mediante polymerase chain reaction con los primers JDP1/JDP2 específicos para género. De las 65 muestras tomadas, 13 fueron positivas a Acanthamoeba spp. Estas fueron caracterizadas a nivel de genotipo mediante la secuenciación del fragmento DF3. Los resultados de la secuenciación revelaron la presencia de los genotipos T4, T5 y T15 dentro de las muestras estudiadas, siendo la más frecuente la T4. Nuestro estudio revela importancia de la presencia de Acanthamoeba en el ambiente ganadero y la necesidad de realizar más estudios, para asociar la presencia de estos organismos y el papel que cumplen en patologías veterinarias. Este es el primer estudio en la provincia de La Pampa que demuestra la presencia de Acanthamoeba y el primero estudiado a nivel de genotipo de Argentina.
Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina. Sample were inoculated onto non-nutrient agar plates and were checked for the presence of Acanthamoeba. Polymerase chain reaction was performed with genus-specific primers JDP1/JDP2, followed by direct sequencing of the polymerase chain reaction product for molecular identification. Sequencing results revealed the presence of T4, T5 and T15 genotypes within the studied samples. Sequencing revealed presence of T4, T5 and T15 in the samples studied genotypes, the most frequent T4. Our study reveals importance of the presence of Acanthamoeba in the livestock environment and the need for further studies to associate the presence of these organisms and the role in veterinary pathology. To the best of our knowledge, this is the first report demonstrating the presence Acanthamoeba in La Pampa province and the first study at the genotype level in Argentina.
Asunto(s)
Microbiología del Agua , Acanthamoeba , Argentina , Acanthamoeba/aislamiento & purificación , Acanthamoeba/genética , Agua , Reacción en Cadena de la Polimerasa , GenotipoRESUMEN
Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina. Sample were inoculated onto non-nutrient agar plates and were checked for the presence of Acanthamoeba. Polymerase chain reaction was performed with genus-specific primers JDP1/JDP2, followed by direct sequencing of the polymerase chain reaction product for molecular identification. Sequencing results revealed the presence of T4, T5 and T15 genotypes within the studied samples. Sequencing revealed presence of T4, T5 and T15 in the samples studied genotypes, the most frequent T4. Our study reveals importance of the presence of Acanthamoeba in the livestock environment and the need for further studies to associate the presence of these organisms and the role in veterinary pathology. To the best of our knowledge, this is the first report demonstrating the presence Acanthamoeba in La Pampa province and the first study at the genotype level in Argentina.
